Significance of endothelin-1 differential expression in sera and CSF of patients with acute craniocerebral inju-ry / 中国基层医药

Objective To elucidate clinical significance of the varied concentration of cytokines in patients , the concentration of endothelin-1(ET-1) in cerebrospinal fluid(CSF) and serum were investigated in 36 patients with acute craniocerebral injury .Methods In this study ,36 patients with acute craniocerebral injury were divided into two groups by their GCS scores ( GCS≤12 as moderate-severe injury group ,GCS>12 as mild injury group ) .Concentration of ET-1 in CSF and serum in 36 patients with acute craniocerebral injury were detected by ELISA .Results The con-centration of ET-1 was(38.89 ±9.50)μg/L in cerebrospinal fluid ( CSF) in moderate to severe patients ,which were significantly higher than that in mild injury groups [(22.25 ±8.55)μg/L](t=5.453,P=0.000) and control groups [(15.67 ±7.72)μg/L](t=8.347,P=0.000).And the levels of ET-1 in CSF in the mild injury groups were also obviously higher than that of the control groups (t=2.390,P=0.023).On the other hand,the concentration of ET-1 was(107.02 ±17.25)μg/L in sera in moderate to severe patients ,which were also significantly higher than that in mild injury groups[(46.21 ±11.19)μg/L](t=12.176,P=0.000)and control groups[(32.34 ±10.64)μg/L] (t=16.163,P=0.000).And the levels of ET-1 in sera in the mild injury groups were obviously higher than that of the control groups(t=3.751,P=0.001).The concentration of ET-1 in sera was significantly higher than that of CSF (t=9.974,P=0.000).Conclusion The concentration of ET-1 in CSF and sera in patients with acute craniocere-bral injury were associated with severe of brain injury .

Inhibitory Effect of CCL20 on CD4+ CD25+ regulatory T cell development in mouse thymus / 中国实验血液学杂志

The aim of this study was to investigate the roles of chemokine CCL20 in development of CD4(+)CD25(+) thymocytes by means of fetal thymus organ culture. Fetal mouse thymus lobes were removed at the fetus age of 14.5 days and cultured in complete RPMI 1640 with 20% FBS in vitro. Phenotypes of the thymocytes were analyzed by FACS and the number of cells per lobe was counted. The results revealed that from day 14.5 to day 19, the absolute and relative numbers of the CD4(+)CD25(+) thymocytes varied similarly as their development as in vitro culture at 6 days. Data showed that during the 6 days in vitro culture the CD4(+)CD25(+) cell percentage out of CD4(+) cells was 58.29%, 12.14%, 6.08%, 17.78%, 9.06%, 4.04% and the CD4(+)CD25(+) cell percentage out of CD25(+) cells was 3.75%, 10.81%, 17.20%, 51.93%, 61.64%, 80.06%. All these data indicated similar characters to their development in vivo. Moreover, at interference with CCL20, the percentage of CD4(+)CD25(+) T cells in thymocytes significantly decreased at the 3 and 6 days from 3.24+/-0.18 and 3.96+/-0.24 to 1.27+/-0.11 (p<0.001) and 1.76+/-0.22 (p<0.001) respectively. It is concluded that the development of CD4(+)CD25(+) thymocytes is similar both in vitro and in vivo, interfering with CCL20 significantly downregulate the expression of CD4(+)CD25(+) T cells. The above data may help to understand the development of naturally arising CD4(+)CD25(+) regulatory T cells.

Expression change in vascular endothelial growth factor in hepatic tissues of hepatitis B patients / 解放军医学杂志

Objective To study the relationship between vascular endothelial growth factor (VEGF) expression and proliferation of hepatic blood vessel and fibrosis in hepatitis B (HB) patients. Methods The total RNA of VEGF was extracted from human liver tissues, and VEGF mRNA probe was acquired by RT-PCR. It was then labeled on hepatic tissues of 160 patients with HB and 10 healthy individuals (control group). Immunohistochemistry of VEGF was performed at the same time. Results The results of hybridization in situ showed that VEGF mRNA was negative in the control group. While in the HB groups, VEGF mRNA was located in the hepatic sinusoids, Disse′s space and hepatocyte cytoplast around the dilated sinusoids. Immunohistochemistry showed that VEGF was expressed in three patterns: the cytoplasm, sinusoid membrane, and sinusoidal endothelium. The expression strength and distribution range of VEGF were closely related with the grading and staging of HB, hepatic vascular inflammation, destruction, obstruction, proliferation and fibrosis. There was remarkable difference between different liver pathological changes (P